Background:

C1 is a non-covalent complex of three different proteins (C1q, C1r and C1s) bound together in a calcium-dependent complex.C1 binds and is activated by antibodies bound to antigens yielding a protease that initiates the classical pathway of complement.

Storage:

-70℃ or below. Avoid freeze/thaw.

Source:

Normal human serum

Purity:

Purified C1 complex is free of other complement proteins, but it contains other high MW plasma proteins.

Molecular Weight:

766,000 Da

Size:

 200 µg/vial

Concentration:

 >0.20 mg C1/mL based on functional activity measurement

Protein Conc.

0.5 to 5.0 mg/mL total protein

Form:

 Frozen liquid

Activity:

more than 250,000 C1H50 units/mg total protein.

Buffer:

10 mM EDTA, 300 mM NaCl, 40% glycerol, pH 5.5, 50 mM sodium acetate, 50 mM EACA, 10 mM benzamidine.

Preservative:

None, 0.22 µm filtered.